Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial Dermatophagoides pteronyssinus Extracts
Identifieur interne : 005778 ( Main/Exploration ); précédent : 005777; suivant : 005779Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial Dermatophagoides pteronyssinus Extracts
Auteurs : Anne Casset [Autriche] ; Adriano Mari [Italie] ; Ashok Purohit [France] ; Yvonne Resch [Autriche] ; Margit Weghofer [Autriche] ; Rosetta Ferrara [Italie] ; Wayne R. Thomas [Australie] ; Claudia Alessandri [Italie] ; Kuan-Wei Chen [Autriche] ; Frederic De Blay [France] ; Rudolf Valenta [Autriche] ; Susanne Vrtala [Autriche]Source :
- International archives of allergy and immunology [ 1018-2438 ] ; 2012.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Allergie, Immunologie.
English descriptors
- KwdEn :
Abstract
Background: Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available Dermatophagoides pteronyssinus extracts from different manufacturers regarding allergen composition and content and whether variations may affect their allergenic activity. Methods: Antibodies specific for several D. pteronyssinus allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 and 21) by dot blot. Results: Only Der p 1 and Der p 2 were detected in all extracts but their concentrations and ratios showed high variability (Der p 1: 6.0-40.8 μg ml-1; Der p 2: 1.7-45.0 μg ml-1). At least 1 out of 4 allergens (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. Mite-allergic subjects showed different IgE reactivity profiles to the individual mite allergens, the extracts showed different allergenic activity in skin-prick tests and false-negative results. Conclusions: Commercially available D. pteronyssinus extracts lack important allergens, show great variability regarding allergen composition and content and some gave false-negative diagnostic test results in certain patients.
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Affiliations:
- Australie, Autriche, France, Italie
- Alsace (région administrative), Grand Est, Latium, Vienne (Autriche)
- Rome, Strasbourg, Vienne (Autriche)
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Thomas</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Center of Child Health Research, University of Western Australia, Telethon Institute for Child Health Research</s1><s2>Perth, W.A</s2><s3>AUS</s3><sZ>7 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Perth, W.A</wicri:noRegion></affiliation></author><author><name sortKey="Alessandri, Claudia" sort="Alessandri, Claudia" uniqKey="Alessandri C" first="Claudia" last="Alessandri">Claudia Alessandri</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Center for Molecular Allergology, IDI-IRCCS</s1><s2>Rome</s2><s3>ITA</s3><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Italie</country><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Chen, Kuan Wei" sort="Chen, Kuan Wei" uniqKey="Chen K" first="Kuan-Wei" last="Chen">Kuan-Wei Chen</name><affiliation wicri:level="3"><inist:fA14 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level="a">Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial Dermatophagoides pteronyssinus Extracts</title><author><name sortKey="Casset, Anne" sort="Casset, Anne" uniqKey="Casset A" first="Anne" last="Casset">Anne Casset</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, and the Christian Doppler Laboratories for, Medical University of Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Mari, Adriano" sort="Mari, Adriano" uniqKey="Mari A" first="Adriano" last="Mari">Adriano Mari</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Center for Molecular Allergology, IDI-IRCCS</s1><s2>Rome</s2><s3>ITA</s3><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Italie</country><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Purohit, Ashok" sort="Purohit, Ashok" uniqKey="Purohit A" first="Ashok" last="Purohit">Ashok Purohit</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Division of Asthma and Allergology, Department of Chest Diseases, Strasbourg University Hospital</s1><s2>Strasbourg</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Grand Est</region><region type="old region">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Resch, Yvonne" sort="Resch, Yvonne" uniqKey="Resch Y" first="Yvonne" last="Resch">Yvonne Resch</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, and the Christian Doppler Laboratories for, Medical University of Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Weghofer, Margit" sort="Weghofer, Margit" uniqKey="Weghofer M" first="Margit" last="Weghofer">Margit Weghofer</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, and the Christian Doppler Laboratories for, Medical University of Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Ferrara, Rosetta" sort="Ferrara, Rosetta" uniqKey="Ferrara R" first="Rosetta" last="Ferrara">Rosetta Ferrara</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Center for Molecular Allergology, IDI-IRCCS</s1><s2>Rome</s2><s3>ITA</s3><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Italie</country><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Thomas, Wayne R" sort="Thomas, Wayne R" uniqKey="Thomas W" first="Wayne R." last="Thomas">Wayne R. Thomas</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Center of Child Health Research, University of Western Australia, Telethon Institute for Child Health Research</s1><s2>Perth, W.A</s2><s3>AUS</s3><sZ>7 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Perth, W.A</wicri:noRegion></affiliation></author><author><name sortKey="Alessandri, Claudia" sort="Alessandri, Claudia" uniqKey="Alessandri C" first="Claudia" last="Alessandri">Claudia Alessandri</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Center for Molecular Allergology, IDI-IRCCS</s1><s2>Rome</s2><s3>ITA</s3><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Italie</country><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Chen, Kuan Wei" sort="Chen, Kuan Wei" uniqKey="Chen K" first="Kuan-Wei" last="Chen">Kuan-Wei Chen</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, and the Christian Doppler Laboratories for, Medical University of Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="De Blay, Frederic" sort="De Blay, Frederic" uniqKey="De Blay F" first="Frederic" last="De Blay">Frederic De Blay</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Division of Asthma and Allergology, Department of Chest Diseases, Strasbourg University Hospital</s1><s2>Strasbourg</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Grand Est</region><region type="old region">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Valenta, Rudolf" sort="Valenta, Rudolf" uniqKey="Valenta R" first="Rudolf" last="Valenta">Rudolf Valenta</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, and the Christian Doppler Laboratories for, Medical University of Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Allergy Research, Medical University of Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>11 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Vrtala, Susanne" sort="Vrtala, Susanne" uniqKey="Vrtala S" first="Susanne" last="Vrtala">Susanne Vrtala</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, and the Christian Doppler Laboratories for, Medical University of Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Development of Allergen Chips, Medical University of Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author></analytic><series><title level="j" type="main">International archives of allergy and immunology</title><title level="j" type="abbreviated">Int. arch. allergy immunol.</title><idno type="ISSN">1018-2438</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">International archives of allergy and immunology</title><title level="j" type="abbreviated">Int. arch. allergy immunol.</title><idno type="ISSN">1018-2438</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Allergen</term><term>Allergy</term><term>Biological activity</term><term>Composition</term><term>Dermatophagoides pteronyssinus</term><term>Extract</term><term>House dust</term><term>Immunology</term><term>Immunopathology</term><term>In vivo</term><term>Mite</term><term>Prick test</term><term>Recombinant protein</term><term>Skin test</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Allergie</term><term>Allergène</term><term>Composition</term><term>In vivo</term><term>Activité biologique</term><term>Dermatophagoides pteronyssinus</term><term>Extrait</term><term>Poussière maison</term><term>Acarien</term><term>Test cutané</term><term>Prick test</term><term>Protéine recombinante</term><term>Immunologie</term><term>Immunopathologie</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Allergie</term><term>Immunologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available Dermatophagoides pteronyssinus extracts from different manufacturers regarding allergen composition and content and whether variations may affect their allergenic activity. Methods: Antibodies specific for several D. pteronyssinus allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 and 21) by dot blot. Results: Only Der p 1 and Der p 2 were detected in all extracts but their concentrations and ratios showed high variability (Der p 1: 6.0-40.8 μg ml<sup>-1</sup>; Der p 2: 1.7-45.0 μg ml<sup>-1</sup>). At least 1 out of 4 allergens (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. Mite-allergic subjects showed different IgE reactivity profiles to the individual mite allergens, the extracts showed different allergenic activity in skin-prick tests and false-negative results. Conclusions: Commercially available D. pteronyssinus extracts lack important allergens, show great variability regarding allergen composition and content and some gave false-negative diagnostic test results in certain patients.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Autriche</li><li>France</li><li>Italie</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li><li>Latium</li><li>Vienne (Autriche)</li></region><settlement><li>Rome</li><li>Strasbourg</li><li>Vienne (Autriche)</li></settlement></list><tree><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Casset, Anne" sort="Casset, Anne" uniqKey="Casset A" first="Anne" last="Casset">Anne Casset</name></region><name sortKey="Chen, Kuan Wei" sort="Chen, Kuan Wei" uniqKey="Chen K" first="Kuan-Wei" last="Chen">Kuan-Wei Chen</name><name sortKey="Resch, Yvonne" sort="Resch, Yvonne" uniqKey="Resch Y" first="Yvonne" last="Resch">Yvonne Resch</name><name sortKey="Valenta, Rudolf" sort="Valenta, Rudolf" uniqKey="Valenta R" first="Rudolf" last="Valenta">Rudolf Valenta</name><name sortKey="Valenta, Rudolf" sort="Valenta, Rudolf" uniqKey="Valenta R" first="Rudolf" last="Valenta">Rudolf Valenta</name><name sortKey="Vrtala, Susanne" sort="Vrtala, Susanne" uniqKey="Vrtala S" first="Susanne" last="Vrtala">Susanne Vrtala</name><name sortKey="Vrtala, Susanne" sort="Vrtala, Susanne" uniqKey="Vrtala S" first="Susanne" last="Vrtala">Susanne Vrtala</name><name sortKey="Weghofer, Margit" sort="Weghofer, Margit" uniqKey="Weghofer M" first="Margit" last="Weghofer">Margit Weghofer</name></country><country name="Italie"><region name="Latium"><name sortKey="Mari, Adriano" sort="Mari, Adriano" uniqKey="Mari A" first="Adriano" last="Mari">Adriano Mari</name></region><name sortKey="Alessandri, Claudia" sort="Alessandri, Claudia" uniqKey="Alessandri C" first="Claudia" last="Alessandri">Claudia Alessandri</name><name sortKey="Ferrara, Rosetta" sort="Ferrara, Rosetta" uniqKey="Ferrara R" first="Rosetta" last="Ferrara">Rosetta Ferrara</name></country><country name="France"><region name="Grand Est"><name sortKey="Purohit, Ashok" sort="Purohit, Ashok" uniqKey="Purohit A" first="Ashok" last="Purohit">Ashok Purohit</name></region><name sortKey="De Blay, Frederic" sort="De Blay, Frederic" uniqKey="De Blay F" first="Frederic" last="De Blay">Frederic De Blay</name></country><country name="Australie"><noRegion><name sortKey="Thomas, Wayne R" sort="Thomas, Wayne R" uniqKey="Thomas W" first="Wayne R." last="Thomas">Wayne R. 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